Antibodies to Glutamic Acid Decarboxylase-65 is Associated with Total Daily Dose of Insulin Requirement in Children with Type 1 Diabetes

BACKGROUND: Type 1 diabetes (T1D) mostly occurs due to the destruction of pancreatic beta cells autoimmune processes. Diagnosis T1D can be established by examining c-peptide levels and markers autoantibodies, including glutamic acid decarboxylase 65 autoantibodies (GAD-65). However, association between anti-GAD-65 toward patients’ clinical manifestations needs further explored. Hence, aim current study was identify anti-GAD65 with characteristics in children T1D.METHODS: Case-control involving 47 (T1D group) 41 healthy (control younger than 18 years old conducted. Secondary data regarding subjects’ demographic medical history were collected from subjects, serum blood drawn each subject for measurement. Anti-GAD65 measured using an Enzyme-Linked Immunosorbent Assay (ELISA) methods.RESULTS: antibody detected 78.7% group, while only 2.43% control group (p=0.0000). The level 0.07±0.19 nmol/L 1.5±0.77 total daily dose insulin subjects positive greater negative (p=0.012). sensitivity specificity 85.4% 66.7%, respectively.CONCLUSION: results this show that associated requirement T1D.KEYWORDS: mellitus, type diabetes, anti-GAD65,